Results cellular models
Characterization of additional cellular models for FOP (Fibrodysplasia ossificans progressiva)
Apart from Hek293 cells, three alternative cell lines were assessed for their use as cellular models of FOP:
- HUVEC (Human umbilical vein endothelial cells)
- U2OS (human osteosarcoma cells)
- Jurkat (immortalized T lymphocytes)
The cell lines were tested for the following parameters:
- Ease of cultivation
- Active BMP signaling pathway
- Efficiency of transfection
Characterization of BMP4 response in U2OS cells
- A comparison between ALK2-expressing Hek293 cells and U2OS cells is shown
- Cells were stimulated with BMP4 and samples were taken at indicated time points
- BMP4 response was assessed using Western blotting with anti phospho-Smad and anti phospho-p38 antibodies
- U2OS show a proper activation of Smad and p38 in response to BMP4 treatment
Summary: characterization of additional cellular models for FOP
Based on the results of the characterization studies, U2OS cells were selected as additional cellular model to study ALK2 interactions