New Search

If you are not happy with the results below please do another search

7 search results for:

1

Analysis using LRC BMP4

Analysis of BMP4 interactions using LRC The ligand-receptor capture technique allows identification of cell surface receptors which interact with a ligand of interest Importantly, LRC allows detection of these interactions on living cells or tissues The ligand is modified with a trifunctionalcrosslinker (TRICEPS) and incubated with target cells Interaction of the ligand with its target…

2

Discussion

The ALK2 interaction network in U2OS cells Summary of ALK2 interactors identified in U2OS cells Discussion: theintracellular ALK2 interaction network in U2OS cells Apart from typical signaling components, the ALK2 interaction network shows a range of regulatory proteins involved in receptor sorting and turnover These include classical chaperones, components of the ubiquitination pathway and the…

3

Analysis U2OS cells

Analysis of ALK2 interactions in U2O Scells To characterize interactions involving ALK2 in U2OS cells, transient transfection was used to express wild type or R206H variants of ALK2 Transiently expressing U2OS were either used unstimulated, or were stimulated with BMP4 Datasets from unstimulated and BMP4 stimulated cells were compared to identify changes in the protein…

4

Results cellular models

Characterization of additional cellular models for FOP (Fibrodysplasia ossificans progressiva) Apart from Hek293 cells, three alternative cell lines were assessed for their use as cellular models of FOP: HUVEC (Human umbilical vein endothelial cells) U2OS (human osteosarcoma cells) Jurkat (immortalized T lymphocytes) The cell lines were tested for the following parameters: Ease of cultivation Active…

5

Results

Investigation of ALK2 interactions in Hek293 cells The following BMP4 signal cascade components were chosen for investigation: ALK2 wild type receptor ALK2 mutated receptors: R206H (causes FOP (Fibrodysplasia ossificans progressiva)) Q207D (constitutive active) K235R (dominant negative) ALK6 wild type receptor ALK6 mutated receptor (I200K): causes brachydactyl (shortness of the fingers and toes) BMPRII: type II…

6

The mechanisms of disturbances

Introduction to Fibrodysplasia ossificans progressiva (FOP) In 2006, a landmark study showed that a point mutation in the ALK2 receptor is linked to FOP (Shore et al., 2006) ACVR1/ALK2 is part of the family of type I BMP receptors (BMPRs) BMPRs play a crucial role in bone formation during development The study suggests that the…

7

Introduction FOP

Introduction to Fibrodysplasia ossificans progressiva (FOP, Münchmeyer syndrome) Fibrodysplasia ossificans progressiva (FOP, Münchmeyer syndrome) First mentioned in England around 1740 Orphan disease, approx. one patient per 2 million individuals Approx. 600 cases are documented Autosomal dominant Average life span: 45 years Early indicator: deformed big toes Progressive incidents of inflammation, followed by differentiation of inflamed…