Characterization of additional cellular models for FOP (Fibrodysplasia ossificans progressiva)

Apart from Hek293 cells, three alternative cell lines were assessed for their use as cellular models of FOP:

  • HUVEC (Human umbilical vein endothelial cells)
  • U2OS (human osteosarcoma cells)
  • Jurkat (immortalized T lymphocytes)

The cell lines were tested for the following parameters:

  • Ease of cultivation
  • Active BMP signaling pathway
  • Efficiency of transfection

Characterization of BMP4 response in U2OS cells

  • A comparison between ALK2-expressing Hek293 cells and U2OS cells is shown
  • Cells were stimulated with BMP4 and samples were taken at indicated time points
  • BMP4 response was assessed using Western blotting with anti phospho-Smad and anti phospho-p38 antibodies
  • U2OS show a proper activation of Smad and p38 in response to BMP4 treatment


Summary: characterization of additional cellular models for FOP


Based on the results of the characterization studies, U2OS cells were selected as additional cellular model to study ALK2 interactions