Characterization of additional cellular models for FOP (Fibrodysplasia ossificans progressiva)
Apart from Hek293 cells, three alternative cell lines were assessed for their use as cellular models of FOP:
- HUVEC (Human umbilical vein endothelial cells)
- U2OS (human osteosarcoma cells)
- Jurkat (immortalized T lymphocytes)
The cell lines were tested for the following parameters:
- Ease of cultivation
- Active BMP signaling pathway
- Efficiency of transfection
Characterization of BMP4 response in U2OS cells
- A comparison between ALK2-expressing Hek293 cells and U2OS cells is shown
- Cells were stimulated with BMP4 and samples were taken at indicated time points
- BMP4 response was assessed using Western blotting with anti phospho-Smad and anti phospho-p38 antibodies
- U2OS show a proper activation of Smad and p38 in response to BMP4 treatment
Summary: characterization of additional cellular models for FOP
Based on the results of the characterization studies, U2OS cells were selected as additional cellular model to study ALK2 interactions